Whole Genome Amplification
The GenomiPhi DNA Amplification kit from GE Healthcare is used to amplify genomic DNA. In this method, bacteriophage Phi29 DNA polymerase enzyme amplifies single or double stranded linear DNA templates exponentially by strand displacement amplification. This system give high fidelity amplification of DNA and is particularly useful in situations where DNA quantities are limiting.
Sample Preparation:
Please provide 1.5ml tubes of 4ng/µl templated DNA in nuclease free water. Sample volume needs to be at least 8µl.
Order Submission:
Please submit the completed order form found here to genomics-core@cgm.northwestern.edu. Please include your chart string or PO number in the email.
Sample Submission:
Please submit samples in secure tubes with clear labels or barcodes.
Freeze samples prior to packing, and ship with sufficient dry ice to ensure they remain frozen. This is to avoid the possibility of cross-contamination or degradation. We cannot accept samples for any study that have thawed or were not shipped frozen.
We recommend that you completely fill the shipping container with dry ice to minimize air space. We also recommend that you pack samples in cardboard freezer boxes placed in large plastic bags to protect samples from dry ice, and use dry ice pellets rather than blocks to avoid damage during shipment.
Include a packing list containing sample IDs, sample type, cell count (if applicable) and box number and position. Please forward an electronic version of this packing list as well so we can have a copy for our records. If necessary, also include any special instructions for the handling of your samples.
There are several ways you may send your samples to the Core.
If you are from Northwestern and are on the Chicago campus, you can bring your samples directly to the facility:
Ward 9-144
303 E Chicago Ave
Chicago IL 60611
If you are from Northwestern and are on the Evanston campus, you can drop your samples off at one of the two courier locations in the Hogan Building, 2205 Tech Drive:
- Hogan 2-100, BMBCB administrative office
- Hogan 4-150
The couriers pick up the samples every morning and we receive them in the afternoon.
If you are not on Northwestern's campus, please send your samples through an independent carrier such as FedEdx or UPS.
Our mailing address:
Genomics Core
303 E Chicago Ave
Ward 9-144
Chicago IL 60611
Turnaround Time:
Services will be completed within 3-4 business following the receipt of your samples.
Results:
After the service is complete, you will receive a username and password to view your results on our server. Results for this service will be delivered as a PDF file with evidence of amplified DNA.
